LoboVault Home

The development of nanofluidic platforms for biomolecular separations


Please use this identifier to cite or link to this item: http://hdl.handle.net/1928/9839

The development of nanofluidic platforms for biomolecular separations

Show simple item record

dc.contributor.author Garcia, Anthony
dc.date.accessioned 2009-08-27T22:10:34Z
dc.date.available 2009-08-27T22:10:34Z
dc.date.issued 2009-08-27T22:10:34Z
dc.date.submitted July 2009
dc.identifier.uri http://hdl.handle.net/1928/9839
dc.description.abstract Recently the extrication and purification of important biomolecules, especially proteins, has been a topic of great interest. Common systems used in the purification and extraction of these important biological molecules includes gel electrophoresis, various forms of chromatography, and capillary electrophoresis type devices. These systems, while achieving a good amount of success, each possess inherent properties that can be improved upon. They usually necessitate comparatively large laboratory footprints, require high voltages to operate (in the tens of kilovolts), and call for relatively large sample sizes in order to perform these tasks. All of these shortcomings can be addressed by performing these separations and purifications in smaller, nanofluidic based systems. This dissertation presents research conducted to explore how fluids, electrical fields, and dissolved analytes of interest (including biomolecules) behave in nanoconfined systems. It presents the recent advances in fabrication that allow for such studies to be embarked upon. It also presents various experimental methodologies for performing investigational work within nanofluidic systems. These include the development of observation techniques, the various methods of controlling nanofluidic transport, buffers, surfactants, and analyte selection. Several techniques for performing separations that are unique to nanofluidic systems are explored. Finally, theoretical analytic models are developed to characterize the nanofluidic transport properties observed in these systems. This work has tendered many groundbreaking advances in the field of separations and in nanofluidics. It will provide the groundwork for any future nanofluidic studies. en_US
dc.description.sponsorship National Science Foundation, Keck Foundation en_US
dc.language.iso en_US en_US
dc.subject Nanofluidics en_US
dc.subject Electrokinetics en_US
dc.subject Lab-on-a-Chip en_US
dc.subject Separations en_US
dc.subject.lcsh Biomolecules--Separation.
dc.subject.lcsh Microfluidics.
dc.subject.lcsh Microfluidic devices.
dc.subject.lcsh Nanotechnology.
dc.title The development of nanofluidic platforms for biomolecular separations en_US
dc.type Dissertation en_US
dc.description.degree Mechanical Engineering en_US
dc.description.level Doctoral en_US
dc.description.department University of New Mexico. Dept. of Mechanical Engineering en_US
dc.description.advisor Mammoli, Andrea
dc.description.advisor Lopez, Gabriel
dc.description.committee-member Truman, C. Randall
dc.description.committee-member Petsev, Dimiter

Files in this item

Files Size Format View
GarciaAL Dissertation Final.pdf 15.99Mb PDF View/Open

This item appears in the following Collection(s)

Show simple item record

UNM Libraries

Search LoboVault


My Account