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Proline's Function in Cold Stress and Osmoregulation in Carrot Tissue Culture Suspensions

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Please use this identifier to cite or link to this item: http://hdl.handle.net/1928/286

Proline's Function in Cold Stress and Osmoregulation in Carrot Tissue Culture Suspensions

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dc.contributor.author Shane, Jackie en_US
dc.date.accessioned 2006-03-11T04:01:53Z
dc.date.available 2006-03-11T04:01:53Z
dc.date.issued 1986
dc.identifier.uri http://hdl.handle.net/1928/286
dc.description.abstract Wild type and high free proline suspension-cultured carrot cell lines were used to determine whether proline influences cold tolerance. In addition, various stressful environments were imposed on the suspensions by varying media ingredients, such as the addition of mannitol and absicisic acid (ABA), in an effort to induce proline production. Results showed that an optimum concentration of proline hardened the control lines and acted as a growth stimulant under normal growth conditions. When both lines were grown at 3 C the high proline mutant line C-63, proved to be the hardiest. When grown at room temperature, C-63 was actually the weaker of the two lines. This relationship indicates that proline directly or indirectly influences cold hardiness in carrot cells. Mannitol, which induces osmotic pressure on plant cells, did not significantly increase proline accumulation. Abscisic acid had a lethal effect on the cells even at 10-4 M. concentrations. Smaller concentration may still induce proline production, but this possibility was not explored. en_US
dc.description.sponsorship University of Illinois en_US
dc.format.extent 37376 bytes
dc.format.extent 1744 bytes
dc.format.extent 14067 bytes
dc.format.mimetype application/msword
dc.format.mimetype text/plain
dc.format.mimetype text/plain
dc.language.iso en_US en_US
dc.subject proline en_US
dc.subject osmoregulation en_US
dc.subject carrot en_US
dc.subject tissue cultures en_US
dc.subject suspension en_US
dc.subject temperature stress en_US
dc.title Proline's Function in Cold Stress and Osmoregulation in Carrot Tissue Culture Suspensions en_US
dc.type Preprint en_US


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