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dc.contributor.authorShane, Jackieen_US
dc.date.accessioned2006-03-11T04:01:53Z
dc.date.available2006-03-11T04:01:53Z
dc.date.issued1986
dc.identifier.urihttp://hdl.handle.net/1928/286
dc.description.abstractWild type and high free proline suspension-cultured carrot cell lines were used to determine whether proline influences cold tolerance. In addition, various stressful environments were imposed on the suspensions by varying media ingredients, such as the addition of mannitol and absicisic acid (ABA), in an effort to induce proline production. Results showed that an optimum concentration of proline hardened the control lines and acted as a growth stimulant under normal growth conditions. When both lines were grown at 3 C the high proline mutant line C-63, proved to be the hardiest. When grown at room temperature, C-63 was actually the weaker of the two lines. This relationship indicates that proline directly or indirectly influences cold hardiness in carrot cells. Mannitol, which induces osmotic pressure on plant cells, did not significantly increase proline accumulation. Abscisic acid had a lethal effect on the cells even at 10-4 M. concentrations. Smaller concentration may still induce proline production, but this possibility was not explored.en_US
dc.description.sponsorshipUniversity of Illinoisen_US
dc.format.extent37376 bytes
dc.format.extent1744 bytes
dc.format.extent14067 bytes
dc.format.mimetypeapplication/msword
dc.format.mimetypetext/plain
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dc.language.isoen_USen_US
dc.subjectprolineen_US
dc.subjectosmoregulationen_US
dc.subjectcarroten_US
dc.subjecttissue culturesen_US
dc.subjectsuspensionen_US
dc.subjecttemperature stressen_US
dc.titleProline's Function in Cold Stress and Osmoregulation in Carrot Tissue Culture Suspensionsen_US
dc.typePreprinten_US


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