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Integrated cellular systems

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Please use this identifier to cite or link to this item: http://hdl.handle.net/1928/17477

Integrated cellular systems

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Title: Integrated cellular systems
Author: Harper, Jason Carl
Advisor(s): Brinker, C. Jeffrey
Committee Member(s): Brozik, Susan M.
Lopez, Gabriel P.
Werner-Washburne, Margaret
Atanassov, Plamen
Department: University of New Mexico. Dept. of Chemical and Nuclear Engineering
Subject(s): sol-gel bioencapsulation
cell-based biosensors
living hybrid biomaterials
cell-directed integration
glycerol modified silanes
orthogonal biodetection
LC Subject(s): Microencapsulation.
Nanostructured materials.
Biosensors.
Degree Level: Doctoral
Abstract: The generation of new three-dimensional (3D) matrices that enable integration of biomolecular components and whole cells into device architectures, without adversely altering their morphology or activity, continues to be an expanding and challenging field of research. This research is driven by the promise that encapsulated biomolecules and cells can significantly impact areas as diverse as biocatalysis, controlled delivery of therapeutics, environmental and industrial process monitoring, early warning of warfare agents, bioelectronics, photonics, smart prosthetics, advanced physiological sensors, portable medical diagnostic devices, and tissue/organ replacement. This work focuses on the development of a fundamental understanding of the biochemical and nanomaterial mechanisms that govern the cell-directed assembly and integration process. It was shown that this integration process relies on the ability of cells to actively develop a pH gradient in response to evaporation induced osmotic stress, which catalyzes silica condensation within a thin 3D volume surrounding the cells, creating a functional bio/nano interface. The mechanism responsible for introducing functional foreign membrane-bound proteins via proteoliposome addition to the silica-lipid-cell matrix was also determined. Utilizing this new understanding, 3D cellular immobilization capabilities were extended using sol-gel matrices endowed with glycerol, trehalose, and media components. The effects of these additives, and the metabolic phase of encapsulated S. cerivisiase cells, on long-term viability and the rate of inducible gene expression was studied. This enabled the entrapment of cells within a novel microfluidic platform capable of simultaneous colorimetric, fluorescent, and electrochemical detection of a single analyte, significantly improving confidence in the biosensor output. As a complementary approach, multiphoton protein lithography was utilized to engineer 3D protein matrices in which to integrate cells and direct their behaviors. This process permits, for the first time, the selection and in situ isolation of a single target cell from a population of cells with mixed phenotypes, and the subsequent monitoring of its behavior, and that of its progeny, under well defined conditions. These techniques promise a new means to integrate biomolecules with nanostructures and macroscale systems, and to manipulate cellular behavior at the individual cell level, having significant implications towards development of practical and robust integrated cellular systems.
Graduation Date: December 2011
URI: http://hdl.handle.net/1928/17477

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