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SNAP-25 is a component of a ubiquitous SNARE complex required for evoked neuroexocytosis in GABAergic neurons

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Please use this identifier to cite or link to this item: http://hdl.handle.net/1928/12881

SNAP-25 is a component of a ubiquitous SNARE complex required for evoked neuroexocytosis in GABAergic neurons

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dc.contributor.author Tafoya, Lawrence C. R.
dc.date.accessioned 2011-07-02T18:07:56Z
dc.date.available 2011-07-02T18:07:56Z
dc.date.issued 2011-07-02
dc.date.submitted May 2011
dc.identifier.uri http://hdl.handle.net/1928/12881
dc.description.abstract The processes of vesicular trafficking and membrane fusion are fundamental to nervous system development and communication among neurons within integrated circuits. The regulated release of several neurotransmitters is dependent on synaptosomal-associated protein 25 kDa (SNAP-25)-containing soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) core complexes. The requirement for this fusogenic machinery in evoked neurosecretion has been repeatedly demonstrated through the use of mutant mouse models and neurotoxin blockades. However, the existence of a functional role for a SNAP-25-containing SNARE complex has not been shown in the major inhibitory neurotransmitter system of the brain, gamma-aminobutyric acid (GABA). To determine whether SNAP-25 participates in the evoked GABAergic neurotransmission, we investigated the expression and function of this protein in inhibitory terminals. In addition, we identified the major SNAP-25 isoform expressed by mature GABAergic neurons. The results presented here provide compelling evidence that SNAP-25 is critical for evoked GABA release and is expressed in the presynaptic terminals of mature GABAergic neurons, consistent with its function as a component of a fundamental core SNARE complex required for stimulus-driven neurotransmission. Furthermore, we conclude that SNAP-25b is the predominant isoform expressed in central inhibitory neurons of the adult brain. en_US
dc.language.iso en_US en_US
dc.subject SNAP-25 presynaptic terminal GABA SNARE glutamatergic synaptic vesicle recycling neurotoxin exocytosis en_US
dc.subject.lcsh Neurons--Physiology.
dc.subject.lcsh Presynaptic receptors.
dc.subject.lcsh Exocytosis.
dc.subject.lcsh GABA.
dc.subject.lcsh Membrane fusion.
dc.title SNAP-25 is a component of a ubiquitous SNARE complex required for evoked neuroexocytosis in GABAergic neurons en_US
dc.type Dissertation en_US
dc.description.degree Biomedical Sciences en_US
dc.description.level Doctoral en_US
dc.description.department University of New Mexico. Biomedical Sciences Graduate Program en_US
dc.description.advisor Wilson, Michael C.
dc.description.committee-member Partridge, Donald
dc.description.committee-member Cunningham, Lee Anna
dc.description.committee-member Deretic, Dusanka


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