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dc.contributor.authorTafoya, Lawrence C. R.
dc.date.accessioned2011-07-02T18:07:56Z
dc.date.available2011-07-02T18:07:56Z
dc.date.issued2011-07-02
dc.date.submittedMay 2011
dc.identifier.urihttp://hdl.handle.net/1928/12881
dc.description.abstractThe processes of vesicular trafficking and membrane fusion are fundamental to nervous system development and communication among neurons within integrated circuits. The regulated release of several neurotransmitters is dependent on synaptosomal-associated protein 25 kDa (SNAP-25)-containing soluble N-ethylmaleimide-sensitive factor attachment protein receptor (SNARE) core complexes. The requirement for this fusogenic machinery in evoked neurosecretion has been repeatedly demonstrated through the use of mutant mouse models and neurotoxin blockades. However, the existence of a functional role for a SNAP-25-containing SNARE complex has not been shown in the major inhibitory neurotransmitter system of the brain, gamma-aminobutyric acid (GABA). To determine whether SNAP-25 participates in the evoked GABAergic neurotransmission, we investigated the expression and function of this protein in inhibitory terminals. In addition, we identified the major SNAP-25 isoform expressed by mature GABAergic neurons. The results presented here provide compelling evidence that SNAP-25 is critical for evoked GABA release and is expressed in the presynaptic terminals of mature GABAergic neurons, consistent with its function as a component of a fundamental core SNARE complex required for stimulus-driven neurotransmission. Furthermore, we conclude that SNAP-25b is the predominant isoform expressed in central inhibitory neurons of the adult brain.en_US
dc.language.isoen_USen_US
dc.subjectSNAP-25 presynaptic terminal GABA SNARE glutamatergic synaptic vesicle recycling neurotoxin exocytosisen_US
dc.subject.lcshNeurons--Physiology.
dc.subject.lcshPresynaptic receptors.
dc.subject.lcshExocytosis.
dc.subject.lcshGABA.
dc.subject.lcshMembrane fusion.
dc.titleSNAP-25 is a component of a ubiquitous SNARE complex required for evoked neuroexocytosis in GABAergic neuronsen_US
dc.typeDissertationen_US
dc.description.degreeBiomedical Sciencesen_US
dc.description.levelDoctoralen_US
dc.description.departmentUniversity of New Mexico. Biomedical Sciences Graduate Programen_US
dc.description.advisorWilson, Michael C.
dc.description.committee-memberPartridge, Donald
dc.description.committee-memberCunningham, Lee Anna
dc.description.committee-memberDeretic, Dusanka


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