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Experimental protocols for and studies of the effects of surface passivation and water isotopes on the gliding speed of microtubules propelled by kinesin-1

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Please use this identifier to cite or link to this item: http://hdl.handle.net/1928/12872

Experimental protocols for and studies of the effects of surface passivation and water isotopes on the gliding speed of microtubules propelled by kinesin-1

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Title: Experimental protocols for and studies of the effects of surface passivation and water isotopes on the gliding speed of microtubules propelled by kinesin-1
Author: Maloney, Roger
Advisor(s): Koch, Steve
Committee Member(s): Dunlap, David
Atlas, Susan
Lidke, Keith
Department: University of New Mexico. Dept. of Physics & Astronomy
Subject(s): kinesin-1
microtubules
gliding motility assay
open science
surface passivation
water isotopes
LC Subject(s): Kinesin.
Microtubules.
Cell motility.
Degree Level: Doctoral
Abstract: This dissertation explores how the kinesin-1 and microtubule system is affected by surface passivation and water isotopes. Surface passivation was found to affect the gliding speed that microtubules exhibit in the gliding motility assay and the lengths of microtubules supported by the passivation. It was also found that gliding speeds of microtubules were very sensitive to temperature changes. Studies changing the water isotope were a first attempt to investigate if changing the solvent changed the osmotic pressure of the solution kinesin and microtubules were in. No osmotic pressure changes were observed, however, the experiments using different isotopes of water did illuminate the possibility that kinesin may be sensitive to viscosity changes in the solvent. This experiment also suggests further experiments that can be specifically designed to probe osmotic pressure changes. This thesis was also the first thesis ever, to the best of the author's knowledge, to be done in a completely open format. All information and notebook entries that are related to it, as well as the thesis itself, can be found on the website OpenWetWare. The thesis can also be found there including all the different versions that went into its editing. The philosophy and process of making data open and accessible to every one is also discussed.
Graduation Date: May 2011
URI: http://hdl.handle.net/1928/12872

Files in this item

Files Size Format View Description
AndyMaloneyDissertation.pdf 24.72Mb PDF View/Open Dissertation
AlphaCasein.mp4 12.02Mb Unknown View/Open
BetaCasein.mp4 9.856Mb Unknown View/Open
KappaCasein.mp4 8.739Mb Unknown View/Open
MixedCasein.mp4 8.888Mb Unknown View/Open
WholeCasein.mp4 18.16Mb Unknown View/Open
Depolymerization.avi 700.6Kb Unknown View/Open
FlowCell.avi 61.77Mb Unknown View/Open
FocusDrift.avi 3.753Mb Unknown View/Open
FSM.mp4 17.37Mb Unknown View/Open
Passivation1.avi 9.896Mb Unknown View/Open
TaxolCrystal.avi 3.476Mb Unknown View/Open

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