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Sex Differences in Heat Shock Protein 72 Expression and Inflammatory Response to Acute Exercise in the Heat.

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Please use this identifier to cite or link to this item: http://hdl.handle.net/1928/11145

Sex Differences in Heat Shock Protein 72 Expression and Inflammatory Response to Acute Exercise in the Heat.

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Title: Sex Differences in Heat Shock Protein 72 Expression and Inflammatory Response to Acute Exercise in the Heat.
Author: Gillum, Trevor
Advisor(s): Schneider, Suzanne
Committee Member(s): Kravitz, Len
Mermier, Christine
Moseley, Pope
Department: University of New Mexico. Division of Physical Performance and Development
Subject(s): Heat shock protein, sex differences, thermoregulation, cytokines, immune function
LC Subject(s): Exercise--Physiological aspects--Sex differences
Body temperature--Regulation--Sex differences
Heat shock proteins--Effect of heat on
Cytokines
Degree Level: Doctoral
Abstract: This study evaluated possible sex differences in intracellular heat shock protein 72 (Hsp72), intracellular cytokines, and extracellular Hsp72 (eHsp72) before and after exercise in the heat. Nine non-heat acclimated women (W) (age 23 ± 3, BF 21 ± 2%, VO2max 58 ± 5 ml/kgFFM/min) and nine non-heat acclimated men (M) (age 25 ± 5, BF 12 ± 5%, VO2max 60 ± 7 ml/kgFFM/min) completed 2 treadmill bouts at 60% VO2max for 60 min in a 42°C, 20% RH environment. The W had normal menstrual cycles and were tested in counterbalanced order during follicular (fol) and luteal (lut) phases. M and W’s duplicate trials were separated by 12 ± 2 days. Blood samples were drawn pre, 0, 1, and 4 hrs post-exercise. Mononucleated cells were analyzed for Hsp72, IL-1ra, IL-6, and TNF-α using flow cytometry. eHsp72 was analyzed using ELISA. In trial 1, Hsp72 content increased in M by 37% 4 hrs post exercise (p<0.05), but did not change significantly in W at any time after exercise. When Hsp72 expression was normalized to baseline, M expressed greater Hsp72 than W (p<0.05) after exercise. Baseline Hsp72 increased by 26% in M from trial 1 to trial 2 (p<0.05), but this effect did not occur in W. eHsp72 did not change after exercise, but there was a main effect for M having higher levels than W (p<0.05). While cytokines did not change during exercise, W consistently expressed less IL-1ra than M (p<0.05). IL-6 and TNF-α were higher in the fol than lut phase at 4 hrs post exercise (p<0.05). Our findings suggest that unacclimated M and W differ in their expression of Hsp72 and eHsp72 after exercise in the heat. M up-regulate Hsp 72 after a single bout of exercise in the heat, which persisted for 12 days, suggesting an acquired cellular thermotolerance. The inhibition of Hsp72 expression in W after exercise could be due to a known effect of estrogen to stabilize the cell membrane or to its action as an anti-oxidant.
Graduation Date: July 2010
URI: http://hdl.handle.net/1928/11145

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