LoboVault Home
 

Th1 Cells That Adoptively Transfer Experimental Hypersensitivity Pneumonitis Are Activated Memory Cells

LoboVault

Please use this identifier to cite or link to this item: http://hdl.handle.net/1928/2375

Th1 Cells That Adoptively Transfer Experimental Hypersensitivity Pneumonitis Are Activated Memory Cells

Show simple item record

dc.contributor.author Schuyler, M
dc.contributor.author Gott, K
dc.contributor.author Edwards, B
dc.date.accessioned 2006-10-30T14:53:33Z
dc.date.available 2006-10-30T14:53:33Z
dc.date.issued 1999-11
dc.identifier.citation Lung. 1999 Nov;177(6):377-89 en_US
dc.identifier.issn 0341-2040
dc.identifier.uri http://hdl.handle.net/1928/2375
dc.description PubMed ID: 10541888; Link to published version: http://www.springerlink.com/content/c4q561qvq4nfeuyd/ en
dc.description.abstract Cultured murine CD4+ T cell lines from Saccharopolyspora rectivirgula-sensitized donors with cytokine secretion characteristics of Th1 cells can adoptively transfer murine experimental hypersensitivity pneumonitis (EHP), whereas Th2 CD4+ cell lines cannot (Cell Immunol 177:169-175, 1997). To assess the differences between these cell lines that may be related to the ability to transfer EHP, we determined cell surface markers that distinguish naive from activated/memory cells that indicate activation and that mediate endothelial adhesion. Both Th1 and Th2 T cell lines are CD4+, CD11a+, ICAM-1+, and L-selectin negative. Th1 cells are CD49d (alpha 4) and LPAM (alpha 4 beta 7) positive, with 32% and 42% of the apparent membrane site density quantitated as the mean molecules of equivalent soluble fluorochromes (MESF) values of unstimulated spleen cells, respectively. Th2 cells are weakly alpha 4 and alpha 4 beta 7 positive, with 15% and 11% of the MESF of unstimulated spleen cells. Th1 cell lines are CD45Rb negative and CD44+, whereas Th2 cell lines are CD45Rb intermediate and CD44-/low. Th1 cells are CD25 (IL-2 receptor) low and Th2 cells CD25 high. We conclude that Th1 cells capable of transfer are activated/memory T cells, and Th2 cells incapable of transfer lack some characteristics of memory/activated T cells (i.e., increase of CD44 and decrease of CD45Rb). Both Th1 and Th2 cell lines express alpha 4 beta 7 and alpha 4 (Th1 > Th2), suggesting that alpha(4) integrin may be important in conferring ability to cells to adoptively transfer EHP. en_US
dc.format.extent 465754 bytes
dc.format.mimetype application/pdf
dc.language.iso en_US en_US
dc.publisher Springer New York; Schuyler, M. en_US
dc.relation.ispartofseries 177 en
dc.relation.ispartofseries 6 en
dc.subject Cell lines en_US
dc.subject Hypersensitivity pneumonitis en_US
dc.subject TH1 cells en_US
dc.subject TH2 cells en_US
dc.title Th1 Cells That Adoptively Transfer Experimental Hypersensitivity Pneumonitis Are Activated Memory Cells en_US
dc.type Article en_US


Files in this item

Files Size Format View
Schuyler-10541888.pdf 454.8Kb PDF View/Open

This item appears in the following Collection(s)

Show simple item record

UNM Libraries

Search LoboVault


Advanced Search

Browse

My Account