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Assessment of the biocompatibility, stability, and suitability of novel thermoresponsive films for the rapid generation of cellular constructs

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Please use this identifier to cite or link to this item: http://hdl.handle.net/1928/12865

Assessment of the biocompatibility, stability, and suitability of novel thermoresponsive films for the rapid generation of cellular constructs

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dc.contributor.author Reed, Jamie
dc.date.accessioned 2011-07-02T17:09:22Z
dc.date.available 2011-07-02T17:09:22Z
dc.date.issued 2011-07-02
dc.date.submitted May 2011
dc.identifier.uri http://hdl.handle.net/1928/12865
dc.description.abstract Stimuli responsive polymers (SRP) are of great interest in the bioengineering community due to their use in applications such as drug delivery and tissue engineering. One example of an SRP is poly(N-isopropyl acrylamide) or pNIPAM. This SRP has the capability of changing its conformation with a slight temperature change: adherent mammalian cells spontaneously release as a confluent cell sheet, which can be harvested for cell sheet engineering purposes. Since its initial use in 1968, many researchers have used pNIPAM to obtain a cell sheet composed of their cell type of interest. The differing protocols used for these diverse cell types, such as the conditions used for cell detachment, and the varying methods used for derivatizing substrates with pNIPAM have all led to conflicting reports on the utility of pNIPAM for cell sheet engineering purposes, as well as the relative cytotoxicity of the polymer. In this work, some of the key inconsistencies in the literature and previously unaddressed challenges when utilizing pNIPAM films are overcome for the purpose of rapid generation of cellular constructs, specifically spheroids. Pertinent characteristics of low temperature detachment are investigated for their effect on the kinetics of cell detachment. In addition, a novel, inexpensive method for obtaining pNIPAM films for mammalian cell detachment, combining pNIPAM with a sol-gel, was optimized and compared to plasma polymerization deposition. Furthermore, proper storage conditions (e.g. temperature and relative humidity) for these films were investigated to increase stability of the films for using tissue culture conditions. To increase the speed of generation of cell sheets, electrospun mats and hydrogels with a high surface area-to-volume ratio were developed. The result is a platform appropriate for the rapid formation of cellular constructs, such as engineered tissues and spheroids for cancer cell research. en_US
dc.language.iso en_US en_US
dc.subject Thermoresponsive en_US
dc.subject pNIPAM en_US
dc.subject Biocompatibility en_US
dc.subject XPS en_US
dc.subject Surface analysis en_US
dc.subject Spheroids en_US
dc.subject.lcsh Acrylimide--Thermal properties.
dc.subject.lcsh Attachment mechanisms (Biology)
dc.subject.lcsh Acrylamide--Biocompatibility.
dc.subject.lcsh Bioengineering--Materials.
dc.title Assessment of the biocompatibility, stability, and suitability of novel thermoresponsive films for the rapid generation of cellular constructs en_US
dc.type Dissertation en_US
dc.description.degree Chemical Engineering en_US
dc.description.level Doctoral en_US
dc.description.department University of New Mexico. Dept. of Chemical and Nuclear Engineering en_US
dc.description.advisor Canavan, Heather
dc.description.committee-member Hedberg-Dirk, Elizabeth
dc.description.committee-member Freyer, James
dc.description.committee-member Wandinger-Ness, Angela


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