LoboVault Home
 

Studies of a cyanine-based biosensor and light-induced antibacterial activities of oligo(phenylene ethynylene)s

LoboVault

Please use this identifier to cite or link to this item: http://hdl.handle.net/1928/12123

Studies of a cyanine-based biosensor and light-induced antibacterial activities of oligo(phenylene ethynylene)s

Show full item record

Title: Studies of a cyanine-based biosensor and light-induced antibacterial activities of oligo(phenylene ethynylene)s
Author: Zhou, Zhijun
Advisor(s): Whitten, David
Committee Member(s): Keller, David
Canavan, Heather
Guo, Hua
Schanze, Kirk
Whitten, David
Department: University of New Mexico. Dept. of Chemistry
Subject(s): biocides
self-assembly
singlet oxygen
Light-induced
Biosensor
cyanine
ope
Degree Level: Doctoral
Abstract: This dissertation has been focusing on two subjects: biosensor development and light-activated antimicrobials. A cyanine-based fluorescent biosensor is developed with high sensitivity to detect the presence and activity of caspase-3/7. We demonstrated that supramolecular self-assembly can be useful for designing biosensors. A series of p-Phenylene Ethynylenes (OPEs) have been synthesized. Further photophysical studies show that these molecules have good singlet oxygen yields. The antimicrobial capability increases dramatically when exposed to UV-365 radiation, though dark biocidal activity can be obtained as well. This phenomenon is probably due to the high yields of singlet oxygen of these OPEs, which oxidizes unsaturated membrane and inner components of bacteria, such as protein, DNA, etc. viii Coupled with our previous work about the interactions of EO-OPE-1s with DOPC/cholesterol vesicles, we believe the biocidal process involves (1) EO-OPE-1s penetrate the bacterial membrane, (2) EO-OPE-1s photosensitize the generation of singlet oxygen and other reactive oxygen species and (3) singlet oxygen and/or reactive oxygen species trigger the cytotoxicity.
Graduation Date: December 2010
URI: http://hdl.handle.net/1928/12123

Files in this item

Files Size Format View
ZZJ-dissertation-FinalVersion1.pdf 6.110Mb PDF View/Open

This item appears in the following Collection(s)

Show full item record

UNM Libraries

Search LoboVault


Advanced Search

Browse

My Account